Enhancer detection in the zebrafish using pseudotyped murine retroviruses.
Identifieur interne : 003F06 ( Main/Exploration ); précédent : 003F05; suivant : 003F07Enhancer detection in the zebrafish using pseudotyped murine retroviruses.
Auteurs : Mary Laplante [Norvège] ; Hiroshi Kikuta ; Melanie König ; Thomas S. BeckerSource :
- Methods (San Diego, Calif.) [ 1046-2023 ] ; 2006.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animal génétiquement modifié (génétique), Animaux, Biologie informatique, Clonage moléculaire, Danio zébré (embryologie), Danio zébré (génétique), Gènes rapporteurs, Génie génétique (), Génie génétique (instrumentation), Génomique (), Retroviridae (génétique), Souris, Transfection (), Transfection (instrumentation), Vecteurs génétiques, Éléments activateurs (génétique).
- MESH :
- embryologie : Danio zébré.
- génétique : Animal génétiquement modifié, Danio zébré, Retroviridae.
- instrumentation : Analyse de séquence d'ADN, Animaux, Biologie informatique, Clonage moléculaire, Gènes rapporteurs, Génie génétique, Génomique, Souris, Transfection, Vecteurs génétiques, Éléments activateurs (génétique).
English descriptors
- KwdEn :
- Animals, Animals, Genetically Modified (genetics), Cloning, Molecular, Computational Biology, Enhancer Elements, Genetic, Genes, Reporter, Genetic Engineering (instrumentation), Genetic Engineering (methods), Genetic Vectors, Genomics (methods), Mice, Retroviridae (genetics), Sequence Analysis, DNA, Transfection (instrumentation), Transfection (methods), Zebrafish (embryology), Zebrafish (genetics).
- MESH :
- embryology : Zebrafish.
- genetics : Animals, Genetically Modified, Retroviridae, Zebrafish.
- instrumentation : Genetic Engineering, Transfection.
- methods : Genetic Engineering, Genomics, Transfection.
- Animals, Cloning, Molecular, Computational Biology, Enhancer Elements, Genetic, Genes, Reporter, Genetic Vectors, Mice, Sequence Analysis, DNA.
Abstract
Vectors based on murine retroviruses are among the most efficient means to insert reporter constructs into the context of a vertebrate chromosome with the aim to visualize cis-regulatory information available to a basal promoter at the site of insertion. In combination with using the zebrafish embryo as a readout for the activity of regulatory elements, enhancer detection becomes a powerful technique for gene discovery and for the mapping of the extent of regulatory domains in a vertebrate genome. Our laboratory has performed the only large-scale enhancer detection screen to date in any vertebrate and we describe in this paper the methods we developed to generate viral particles, to insert reporter constructs into the zebrafish germ line, the screening of detection events in heterozygous F1 embryos, and the isolation of genomic sequence flanking the inserted vector for the purpose of genomic mapping. Given sufficient scale, the technology described here can be used to obtain cis-regulatory information across the entire zebrafish genome for any given basal promoter.
DOI: 10.1016/j.ymeth.2006.01.003
PubMed: 16887366
Affiliations:
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Le document en format XML
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Becker</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16887366</idno><idno type="pmid">16887366</idno><idno type="doi">10.1016/j.ymeth.2006.01.003</idno><idno type="wicri:Area/PubMed/Corpus">002142</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002142</idno><idno type="wicri:Area/PubMed/Curation">002142</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002142</idno><idno type="wicri:Area/PubMed/Checkpoint">002205</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002205</idno><idno type="wicri:Area/Ncbi/Merge">001602</idno><idno type="wicri:Area/Ncbi/Curation">001602</idno><idno type="wicri:Area/Ncbi/Checkpoint">001602</idno><idno type="wicri:doubleKey">1046-2023:2006:Laplante M:enhancer:detection:in</idno><idno type="wicri:Area/Main/Merge">004105</idno><idno type="wicri:Area/Main/Curation">003F06</idno><idno type="wicri:Area/Main/Exploration">003F06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Enhancer detection in the zebrafish using pseudotyped murine retroviruses.</title><author><name sortKey="Laplante, Mary" sort="Laplante, Mary" uniqKey="Laplante M" first="Mary" last="Laplante">Mary Laplante</name><affiliation wicri:level="1"><nlm:affiliation>Sars Centre for Marine Molecular Biology, University of Bergen, High Technology Centre, Thormoehlensgate 55, 5008 Bergen, Norway.</nlm:affiliation><country xml:lang="fr">Norvège</country><wicri:regionArea>Sars Centre for Marine Molecular Biology, University of Bergen, High Technology Centre, Thormoehlensgate 55, 5008 Bergen</wicri:regionArea><wicri:noRegion>5008 Bergen</wicri:noRegion></affiliation></author><author><name sortKey="Kikuta, Hiroshi" sort="Kikuta, Hiroshi" uniqKey="Kikuta H" first="Hiroshi" last="Kikuta">Hiroshi Kikuta</name></author><author><name sortKey="Konig, Melanie" sort="Konig, Melanie" uniqKey="Konig M" first="Melanie" last="König">Melanie König</name></author><author><name sortKey="Becker, Thomas S" sort="Becker, Thomas S" uniqKey="Becker T" first="Thomas S" last="Becker">Thomas S. Becker</name></author></analytic><series><title level="j">Methods (San Diego, Calif.)</title><idno type="ISSN">1046-2023</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Animals, Genetically Modified (genetics)</term><term>Cloning, Molecular</term><term>Computational Biology</term><term>Enhancer Elements, Genetic</term><term>Genes, Reporter</term><term>Genetic Engineering (instrumentation)</term><term>Genetic Engineering (methods)</term><term>Genetic Vectors</term><term>Genomics (methods)</term><term>Mice</term><term>Retroviridae (genetics)</term><term>Sequence Analysis, DNA</term><term>Transfection (instrumentation)</term><term>Transfection (methods)</term><term>Zebrafish (embryology)</term><term>Zebrafish (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animal génétiquement modifié (génétique)</term><term>Animaux</term><term>Biologie informatique</term><term>Clonage moléculaire</term><term>Danio zébré (embryologie)</term><term>Danio zébré (génétique)</term><term>Gènes rapporteurs</term><term>Génie génétique ()</term><term>Génie génétique (instrumentation)</term><term>Génomique ()</term><term>Retroviridae (génétique)</term><term>Souris</term><term>Transfection ()</term><term>Transfection (instrumentation)</term><term>Vecteurs génétiques</term><term>Éléments activateurs (génétique)</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Danio zébré</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Zebrafish</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Animals, Genetically Modified</term><term>Retroviridae</term><term>Zebrafish</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Animal génétiquement modifié</term><term>Danio zébré</term><term>Retroviridae</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Genetic Engineering</term><term>Transfection</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Engineering</term><term>Genomics</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cloning, Molecular</term><term>Computational Biology</term><term>Enhancer Elements, Genetic</term><term>Genes, Reporter</term><term>Genetic Vectors</term><term>Mice</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Biologie informatique</term><term>Clonage moléculaire</term><term>Gènes rapporteurs</term><term>Génie génétique</term><term>Génomique</term><term>Souris</term><term>Transfection</term><term>Vecteurs génétiques</term><term>Éléments activateurs (génétique)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Vectors based on murine retroviruses are among the most efficient means to insert reporter constructs into the context of a vertebrate chromosome with the aim to visualize cis-regulatory information available to a basal promoter at the site of insertion. In combination with using the zebrafish embryo as a readout for the activity of regulatory elements, enhancer detection becomes a powerful technique for gene discovery and for the mapping of the extent of regulatory domains in a vertebrate genome. Our laboratory has performed the only large-scale enhancer detection screen to date in any vertebrate and we describe in this paper the methods we developed to generate viral particles, to insert reporter constructs into the zebrafish germ line, the screening of detection events in heterozygous F1 embryos, and the isolation of genomic sequence flanking the inserted vector for the purpose of genomic mapping. Given sufficient scale, the technology described here can be used to obtain cis-regulatory information across the entire zebrafish genome for any given basal promoter.</div></front></TEI><affiliations><list><country><li>Norvège</li></country></list><tree><noCountry><name sortKey="Becker, Thomas S" sort="Becker, Thomas S" uniqKey="Becker T" first="Thomas S" last="Becker">Thomas S. Becker</name><name sortKey="Kikuta, Hiroshi" sort="Kikuta, Hiroshi" uniqKey="Kikuta H" first="Hiroshi" last="Kikuta">Hiroshi Kikuta</name><name sortKey="Konig, Melanie" sort="Konig, Melanie" uniqKey="Konig M" first="Melanie" last="König">Melanie König</name></noCountry><country name="Norvège"><noRegion><name sortKey="Laplante, Mary" sort="Laplante, Mary" uniqKey="Laplante M" first="Mary" last="Laplante">Mary Laplante</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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